Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 28, Pages E2562-E2571Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1303035110
Keywords
protein motor; single molecule biophysics; DNA-end processing; real-time measurements; protein-DNA interactions
Categories
Funding
- European Research Council [206117]
- Spanish Ministry of Economy and Competitiveness [FIS2011-24638]
- Royal Society University Research Fellowship
- Biotechnology and Biological Sciences Research Council Studentship
- Juan de la Cierva [Spanish Ministry of Science and Innovation (MICINN)] [JCI-2011-10277]
- Spanish National Research Council [I-LINK0331]
- European Research Council (ERC) [206117] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
Double-stranded DNA break repair by homologous recombination is initiated by resection of free DNA ends to produce a 3'-ssDNA overhang. In bacteria, this reaction is catalyzed by helicase-nuclease complexes such as AddAB in a manner regulated by specific recombination hotspot sequences called Crossover hotspot instigator (Chi). We have used magnetic tweezers to investigate the dynamics of AddAB translocation and hotspot scanning during double-stranded DNA break resection. AddAB was prone to stochastic pausing due to transient recognition of Chi-like sequences, unveiling an antagonistic relationship between DNA translocation and sequence-specific DNA recognition. Pauses at bona fide Chi sequences were longer, were nonexponentially distributed, and resulted in an altered velocity upon restart of translocation downstream of Chi. We propose a model for the recognition of Chi sequences to explain the origin of pausing during failed and successful hotspot recognition.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available