Journal
JOURNAL OF PLANT INTERACTIONS
Volume 10, Issue 1, Pages 132-141Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/17429145.2015.1036140
Keywords
pathogenesis-related protein 5; Puccinia triticina; adult plant resistance; expression profiles; western blotting
Categories
Funding
- Natural Science Foundation of Hebei Province [C2012204005]
- National Key Basic Research Program of China [2013CB127700]
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TaLr35PR5 gene was obtained from the gDNA and cDNA of TcLr35 wheat. It was induced by Puccinia triticina, ABA and SA, but TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction. In addition, the accumulations of TaLr35PR5 increased stably and showed significant peak challenged by P. triticina at different growth and development periods of TcLr35 wheat while it maintained similar level and changed little in mock inoculated. Western blottings were conducted to confirm that TaLr35PR5 be induced by P. triticina infection at the protein expression level. Similar to the expression patterns of TaLr35PR5 at RNA levels, the accumulations of TaLr35PR5 protein were weak in the seedling stage, then increased to the peak and kept constant levels at the mature stage which is consistent with the expression feature of Lr35 gene as an adult plant resistance gene.
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