Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 109, Issue 23, Pages 8954-8958Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1200966109
Keywords
bacteriophage; crystal structure; Lactococcus lactis; siphoviridae; viral infection
Categories
Funding
- Agence Nationale de la Recherche [ANR BLAN07-1_191968, ANR-11-BSV8-004-01]
- Ministere Francais de l'Enseignement Superieur et de la Recherche [22976-2006]
- Science Foundation Ireland [08/IN.1/B1909]
- Science Foundation Ireland (SFI) [08/IN.1/B1909] Funding Source: Science Foundation Ireland (SFI)
Ask authors/readers for more resources
Phages of the Caudovirales order possess a tail that recognizes the host and ensures genome delivery upon infection. The X-ray structure of the approximately 1.8 MDa host adsorption device (baseplate) from the lactococcal phage TP901-1 shows that the receptor-binding proteins are pointing in the direction of the host, suggesting that this organelle is in a conformation ready for host adhesion. This result is in marked contrast with the lactococcal phage p2 situation, whose baseplate is known to undergo huge conformational changes in the presence of Ca2+ to reach its active state. In vivo infection experiments confirmed these structural observations by demonstrating that Ca2+ ions are required for host adhesion among p2-like phages (936-species) but have no influence on TP901-1-like phages (P335-species). These data suggest that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available