Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 109, Issue 22, Pages 8705-8709Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1201830109
Keywords
tissue regeneration; pluripotent stem cells
Categories
Funding
- National Institutes of Health [DE016525, EB002520]
- New York Stem Cell Foundation
- Slovenian Research Agency [P3-0371]
- Bia and Slovene Human Resources Development and Scholarship Fund
- Aragon Government
- Aragon Health Research Institute
- [CU09-3055]
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In extensive bone defects, tissue damage and hypoxia lead to cell death, resulting in slow and incomplete healing. Human embryonic stem cells (hESC) can give rise to all specialized lineages found in healthy bone and are therefore uniquely suited to aid regeneration of damaged bone. We show that the cultivation of hESC-derived mesenchymal progenitors on 3D osteoconductive scaffolds in bioreactors with medium perfusion leads to the formation of large and compact bone constructs. Notably, the implantation of engineered bone in immunodeficient mice for 8 wk resulted in the maintenance and maturation of bone matrix, without the formation of teratomas that is consistently observed when undifferentiated hESCs are implanted, alone or in bone scaffolds. Our study provides a proof of principle that tissue-engineering protocols can be successfully applied to hESC progenitors to grow bone grafts for use in basic and translational studies.
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