Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 109, Issue 33, Pages 13446-13451Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1119992109
Keywords
cross-regulation; growth
Categories
Funding
- Consejo Superior de Investigaciones Cientificas Fellowship of the Joint Admissions Exercise Predoctoral Program
- Spanish Ministry of Science and Innovation [BIO2007-60923, BIO2010-15071, CSD2007-00057]
- Italian Ministry of Education, University, and Research
- Generalitat Valenciana Grants [ACOMP/2010/190, PROMETEO/2010/020]
- Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom
- BBSRC [BB/E022618/1, BB/E006922/1, BBS/E/C/00005202] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E006922/1, BBS/E/C/00005202, BB/E022618/1] Funding Source: researchfish
Ask authors/readers for more resources
Plant development is modulated by the convergence of multiple environmental and endogenous signals, and the mechanisms that allow the integration of different signaling pathways is currently being unveiled. A paradigmatic case is the concurrence of brassinosteroid (BR) and gibberellin (GA) signaling in the control of cell expansion during photomorphogenesis, which is supported by physiological observations in several plants but for which no molecular mechanism has been proposed. In this work, we show that the integration of these two signaling pathways occurs through the physical interaction between the DELLA protein GAI, which is a major negative regulator of the GA pathway, and BRASSINAZOLE RESISTANT1 (BZR1), a transcription factor that broadly regulates gene expression in response to BRs. We provide biochemical evidence, both in vitro and in vivo, indicating that GAI inactivates the transcriptional regulatory activity of BZR1 upon their interaction by inhibiting the ability of BZR1 to bind to target promoters. The physiological relevance of this interaction was confirmed by the observation that the dominant gai-1 allele interferes with BR-regulated gene expression, whereas the bzr1-1D allele displays enhanced resistance to DELLA accumulation during hypocotyl elongation. Because DELLA proteins mediate the response to multiple environmental signals, our results provide an initial molecular framework for the integration with BRs of additional pathways that control plant development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available