Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 109, Issue 16, Pages 6094-6099Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1201288109
Keywords
cryo-EM; E. coli; ribosome; translation
Categories
Funding
- Spanish Government [RYC-2009-04885]
- Department of Education, Universities and Research of the Basque Country [PI2011-23]
- Howard Hughes Medical Institute
- National Institutes of Health [R01 GM55440, R37 GM29169, R01 GM059425, P41-RR005969]
- National Science Foundation [PHY0822613]
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Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.
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