Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 109, Issue 19, Pages 7196-7201Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1202762109
Keywords
desulfurization; solid phase peptide synthesis
Categories
Funding
- National Institutes of Health [CA28824]
- Sloan-Kettering Institute
- [T32 CA062948-15]
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Nonglycosylated erythropoietin bearing acetamidomethyl protecting groups at the cysteine residues has been synthesized via chemical methods. Alanine ligation was used to assemble four peptide fragments, themselves prepared by solid phase peptide synthesis. This work outlines a route for the synthesis of homogeneous glycosylated erythropoietin.
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