Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 38, Pages 16038-16043Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1111650108
Keywords
-
Categories
Funding
- Gates Grand Challenges Exploration
- European Research Council [24271, ANR-09-JCJC-0118]
- Agence Nationale de la Recherche (ANR) [ANR-09-JCJC-0118] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection and dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias and lower organ titers. These results indicate that increased replication fidelity and reduced genetic diversity negatively impact arbovirus fitness in invertebrate and vertebrate hosts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available