Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 43, Pages 17610-17614Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1108685108
Keywords
DNA oligonucleotide; ruthenium complex; X-ray crystal structure
Categories
Funding
- Royal Society
- Royal Irish Academy
- Diamond Light Source, Ltd.
- University of Reading
- Science Foundation Ireland [06/RF/CHP035]
- Irish Research Council for Science Engineering and Technology
- European Cooperation in Science and Technology
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We describe a crystal structure, at atomic resolution (1.1 angstrom, 100 K), of a ruthenium polypyridyl complex bound to duplex DNA, in which one ligand acts as a wedge in the minor groove, resulting in the 51 degrees kinking of the double helix. The complex cation Lambda-[Ru(1,4,5,8-tetraazaphenanthrene)(2)(dipyridophenazine)](2+) crystallizes in a 1: 1 ratio with the oligonucleotide d(TCGGCGCCGA) in the presence of barium ions. Each complex binds to one duplex by intercalation of the dipyridophenazine ligand and also by semi-intercalation of one of the orthogonal tetraazaphenanthrene ligands into a second symmetrically equivalent duplex. The result is noncovalent cross-linking and marked kinking of DNA.
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