Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 7, Pages 2777-2782Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1100735108
Keywords
fluorescent Ca2+ probe; intracellular organelle tethering; fluorescence resonance energy transfer
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Funding
- Fondo per gli Investimenti della Ricerca di Base (FIRB) [RBIN042Z2Y]
- Italian Ministry of University and Research
- Veneto Region
- Italian Institute of Technology
- University of Padua
- Fondazione Cassa di Risparmio di Padova e Rovigo (CARIPARO) Foundation
- CARIPARO Foundation
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Presenilin mutations are the main cause of familial Alzheimer's disease (FAD). Presenilins also play a key role in Ca2+ homeostasis, and their FAD-linked mutants affect cellular Ca2+ handling in several ways. We previously have demonstrated that FAD-linked presenilin 2 (PS2) mutants decrease the Ca2+ content of the endoplasmic reticulum (ER) by inhibiting sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) activity and increasing ER Ca2+ leak. Here we focus on the effect of presenilins on mitochondrial Ca2+ dynamics. By using genetically encoded Ca2+ indicators specifically targeted to mitochondria (aequorin- and GFP-based probes) in SH-SY5Y cells and primary neuronal cultures, we show that overexpression or down-regulation of PS2, but not of presenilin 1 (PS1), modulates the Ca2+ shuttling between ER and mitochondria, with its FAD mutants strongly favoring Ca2+ transfer between the two organelles. This effect is not caused by a direct PS2 action on mitochondrial Ca2+-uptake machinery but rather by an increased physical interaction between ER and mitochondria that augments the frequency of Ca2+ hot spots generated at the cytoplasmic surface of the outer mitochondrial membrane upon stimulation. This PS2 function adds further complexity to the multifaceted nature of presenilins and to their physiological role within the cell. We also discuss the importance of this additional effect of FAD-linked PS2 mutants for the understanding of FAD pathogenesis.
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