Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 22, Pages 9078-9083Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105682108
Keywords
transient protein unfolding; protein folding intermediate; protein folding mechanism
Categories
Funding
- National Institutes of Health [DK34909]
- Skaggs Institute for Chemical Biology
- Ruth L. Kirschstein [GM84594]
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Detailed understanding of protein function and malfunction hinges on the ability to characterize transiently populated states and the transitions between them. Here, we use (15)N, (1)HN, and (13)CO NMR R(2) relaxation dispersion to investigate spontaneous unfolding and refolding events of native apomyoglobin. Above pH 5.0, dispersion is dominated by processes involving fluctuations of the F-helix region, which is invisible in NMR spectra. Measurements of R2 dispersion for residues contacted by the F-helix region in the native (N) structure reveal a transient state formed by local unfolding of helix F and undocking from the protein core. A similar state was detected at pH 4.75-4.95 and determined to be an on-pathway intermediate (I1) in a linear three-state unfolding scheme (N reversible arrow I1 reversible arrow MG) leading to a transiently populated molten globule (MG) state. The slowest steps in unfolding and refolding are N -> I1 (36 s(-1)) and MG -> I1 (26 s(-1)), respectively. Differences in chemical shift between N and I1 are very small, except in regions adjacent to helix F, showing that their core structures are similar. Chemical shift changes between the N and MG states, obtained from R(2) dispersion, reveal that the transient MG state is structurally similar to the equilibrium MG observed previously at high temperature and low pH. Analysis of MG state chemical shifts shows the location of residual helical structure in the transient intermediate and identifies regions that unfold or rearrange into nonnative structure during the N -> MG transition. The experiments also identify regions of energetic frustration that crack during unfolding and impede the refolding process.
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