Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 26, Pages 10502-10507Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105645108
Keywords
posttranscriptional modification; tRNA processing; subcellular localization; RNA mass spectrometry
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Funding
- Ministry of Education, Science, Sports, and Culture of Japan
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [22227006, 23655153] Funding Source: KAKEN
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The retrograde movement of tRNAs from the cytoplasm to the nucleus occurs constitutively in eukaryotic cells but its functional significance remains unclear. We show evidence suggesting that in Saccharomyces cerevisiae, a spliced tRNA precursor must be imported into the nucleus before the biogenesis of a modified base can occur. Wybutosine (yW) is a modified base adjacent to the anticodon of tRNA(Phe) and is required for accurate decoding. Glucose starvation or overexpression of the nuclear tRNA binding protein Trz1p both caused nuclear retention of cytoplasmic tRNAs, impaired the yW synthesis, and induced the accumulation of its intermediate, N(1)-methylgunanosine (m(1)G), showing that the post-spliced tRNA(Phe) is imported to the nucleus, where m(1)G is formed by Trm5p, after which it is reexported to the cytoplasm, where the yW synthesis is completed by cytoplasmic enzymes.
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