Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 49, Pages 19504-19509Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1109111108
Keywords
-
Categories
Funding
- European doctoral school Frontieres du Vivant
- Fondation pour la Recherche Medicale
- Paris School of Neuroscience
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche
- European Union Strep Photolysis [LSHM-CT-2007-037765]
- Human Frontier Science Program [RGP0013/2010]
- Fondation pour la Recherche Medicale (FRM equipe)
- Medical Research Council [U117512659] Funding Source: researchfish
Ask authors/readers for more resources
Access to three-dimensional structures in the brain is fundamental to probe signal processing at multiple levels, from integration of synaptic inputs to network activity mapping. Here, we present an optical method for independent three-dimensional photoactivation and imaging by combination of digital holography with remote-focusing. We experimentally demonstrate compensation of spherical aberration for out-of-focus imaging in a range of at least 300 mu m, as well as scanless imaging along oblique planes. We apply this method to perform functional imaging along tilted dendrites of hippocampal pyramidal neurons in brain slices, after photostimulation by multiple spots glutamate uncaging. By bringing extended portions of tilted dendrites simultaneously in-focus, we monitor the spatial extent of dendritic calcium signals, showing a shift from a widespread to a spatially confined response upon blockage of voltage-gated Na+ channels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available