Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 21, Pages 8885-8890Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105564108
Keywords
diabetes; secretory granule; endoribonuclease
Categories
Funding
- National Institutes of Health [AI32412, DK082448]
- American Heart Association
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As a key regulator of the unfolded protein response, the transcription factor XBP1 activates genes in protein secretory pathways and is required for the development of certain secretory cells. To elucidate the function of XBP1 in pancreatic beta-cells, we generated beta-cell-specific XBP1 mutant mice. Xbp1(f/f);RIP-cre mice displayed modest hyperglycemia and glucose intolerance resulting from decreased insulin secretion from beta-cells. Ablation of XBP1 markedly decreased the number of insulin granules in beta-cells, impaired proinsulin processing, increased the serum proinsulin: insulin ratio, blunted glucose-stimulated insulin secretion, and inhibited cell proliferation. Notably, XBP1 deficiency not only compromised the endoplasmic reticulum stress response in beta-cells but also caused constitutive hyperactivation of its upstream activator, IRE1 alpha, which could degrade a subset of mRNAs encoding proinsulin-processing enzymes. Hence, the combined effects of XBP1 deficiency on the canonical unfolded protein response and its negative feedback activation of IRE1 alpha caused beta-cell dysfunction in XBP1 mutant mice. These results demonstrate that IRE1 alpha has dual and opposing roles in beta-cells, and that a precisely regulated feedback circuit involving IRE1 alpha and its product XBP1s is required to achieve optimal insulin secretion and glucose control.
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