Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 46, Pages 18714-18719Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1114854108
Keywords
biomaterials; induced pluripotent stem cells; surface science; embryonic stem cells
Categories
Funding
- Howard Hughes Medical Institute
- National Institutes of Health [R37-CA084198, RO1-CA087869, RO1-HD045022, DE016516]
- European Leukodystrophy Association
- Wellcome Trust [085246]
- Society in Science: The Branco-Weiss Fellowship
- Engineering and Physical Sciences Research Council [EP/H045384/1] Funding Source: researchfish
- EPSRC [EP/H045384/1] Funding Source: UKRI
Ask authors/readers for more resources
The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell migration, and results in significant increases in cell growth of undifferentiated cells. These chemically defined xeno-free substrates generate more than three times the number of cells than feeder-containing substrates per surface area. Further, reprogramming and typical gene-targeting protocols can be readily performed on these engineered surfaces. These substrates provide an attractive cell culture platform for the production of clinically relevant factor-free reprogrammed cells from patient tissue samples and facilitate the definition of standardized scale-up friendly methods for disease modeling and cell therapeutic applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available