Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 4, Pages 1711-1716Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1018359108
Keywords
Arabidopsis; stress tolerance; hydrogen peroxide
Categories
Funding
- Ghent University [01MRB510W, 12051403]
- Research Foundation-Flanders [G.0350.04N]
- Scientific Exchange Program Flanders-France [Tournesol T.2008.21]
- National Science Foundation [IOS-0743954, IOS-0950040]
- European Union [447]
- Nevada Agricultural Experimental Station
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1132176] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1132326] Funding Source: National Science Foundation
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Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and peroxisomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H2O2-induced DNA damage. In apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.
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