Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 17, Pages 7102-7106Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1019519108
Keywords
baculovirus expression system; speciation
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology [19208005, 19688004, 22128004]
- Grants-in-Aid for Scientific Research [19208005, 23248008] Funding Source: KAKEN
Ask authors/readers for more resources
(E)-11- and (Z)-11-tetradecenyl acetate are the most common female sex pheromone components in Ostrinia moths. The Delta 11-desaturase expressed in the pheromone gland (PG) of female moths is a key enzyme that introduces a double bond into pheromone molecules. A single Delta 11-desaturase of Ostrinia nubilalis, OnubZ/E11, has been shown to produce an similar to 7: 3 mixture of (E)-11- and (Z)-11-tetradecenoate from the substrate tetradecanoate. In contrast, the sex pheromone of Ostrinia latipennis, a primitive species of Ostrinia, is (E)-11-tetradecenol. This pheromone is unique in that it is not acetylated, and includes no Z isomer. In the present study, through the cloning and functional analysis of a PG-specific Delta 11-desaturase in O. latipennis, we showed that the absence of the Z isomer in the pheromone is attributable to the strict product specificity of the Delta 11-desaturase in this species, LATPG1. Phylogenetic analysis revealed that LATPG1 was not closely related to OnubZ/E11. Rather, it was closely related to retroposon-linked cryptic Delta 11-desaturases (ezi-Delta 11) found in the genomes of O. nubilalis and Ostrinia furnacalis. Taken together, the results showed that an unusual Delta 11-desaturase is functionally expressed in O. latipennis, although the genes encoding this enzyme appear to be cryptic in congeners.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available