Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 31, Pages 12863-12868Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105002108
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Funding
- Diabetes Research Institute Foundation
- National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases/National Institute of Allergy and Infectious Diseases [5U19AI050864-10, 1F32DK083226, R03DK075487, R01DK084321, U01DK089538, M01RR16587, 5U01DK70460-07, R01DK55347-IU42RR016603]
- General Clinical Research Center
- Juvenile Diabetes Research Foundation [4-2004-361, 4-2008-811, 17-2010-5]
- Karolinska Institutet
- Swedish Research Council
- Swedish Diabetes Foundation
- Family Erling-Persson Foundation
- Family Knut and Alice Wallenberg Foundation
- Skandia Insurance Company Ltd.
- In Vivo Imaging of Beta-cell Receptors by Applied Nano Technology [FP7-228933-2]
- Novo Nordisk Foundation
- Berth von Kantzow's Foundation
- Novo Nordisk Fonden [NNF11OC1014706] Funding Source: researchfish
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Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.
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