Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 20, Pages 9192-9197Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1001195107
Keywords
dynamics; riboswitches; ultrafast NMR; conformational transition
Categories
Funding
- Israel Science Foundation (ISF) [447/09]
- European Commission (EU) [026145]
- Helen and Kimmel Award
- Perlman Family Foundation
- National Institutes of Health National Institute of Biomedical Imaging and Bioengineering
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Conformational transitions and structural rearrangements are central to the function of many RNAs yet remain poorly understood. We have used ultrafast multidimensional NMR techniques to monitor the adenine-induced folding of an adenine-sensing riboswitch in real time, with nucleotide-resolved resolution. By following changes in 2D spectra at rates of approximately 0.5 Hz, we identify distinct steps associated with the ligand-induced folding of the riboswitch. Following recognition of the ligand, long range loop-loop interactions form and are then progressively stabilized before the formation of a fully stable complex over approximately 2-3 minutes. The application of these ultrafast multidimensional NMR methods provides the opportunity to determine the structure of RNA folding intermediates and conformational trajectories.
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