Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 19, Pages 8525-8530Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0914794107
Keywords
fluorescent sensing; NO; olfaction; trappable probe; fluorescence microscopy
Categories
Funding
- National Science Foundation [CHE-061194]
- National Institute on Deafness and Other Communication Disorders of the National Institutes of Health [DC-004208]
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We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu(2)(FL2E) and the membrane-impermeable acid derivative Cu(2)(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu(2)(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.
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