Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 37, Pages 16072-16077Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1006026107
Keywords
mitochondrion; RNA polymerase
Categories
Funding
- Swedish Research Council
- Swedish Cancer Society
- European Commission
- Swedish Strategic Foundation
- Goran Gustafsson Foundation
- Knut and Alice Wallenberg Foundation
- Medical Research Council
Ask authors/readers for more resources
The human mitochondrial transcription machinery generates the primers required for initiation of leading-strand DNA replication. According to one model, the 3' end of the primer is defined by transcription termination at conserved sequence block II (CSB II) in the mitochondrial DNA control region. We here demonstrate that this site-specific termination event is caused by G-quadruplex structures formed in nascent RNA upon transcription of CSB II. We also demonstrate that a poly-dT stretch downstream of CSB II has a modest stimulatory effect on the termination efficiency. The mechanism is reminiscent of Rho-independent transcription termination in prokaryotes, with the exception that a G-quadruplex structure replaces the hairpin loop formed in bacterial mRNA during transcription of terminator sequences.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available