Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 33, Pages 14799-14804Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1004204107
Keywords
wound healing; microbiome; innate immunity; diabetes; gene expression
Categories
Funding
- National Human Genome Research Institute Microarray Core
- National Human Genome Research Institute
- National Institute of Diabetes and Digestive and Kidney Diseases [1R56DK080672-01A1, 1P2DK083085-02]
- National Institute of General Medical Sciences Pharmacology Research Associate
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Diabetics frequently suffer from chronic, nonhealing wounds. Although bacterial colonization and/or infection are generally acknowledged to negatively impact wound healing, the precise relationship between the microbial community and impaired wound healing remains unclear. Because the host cutaneous defense response is proposed to play a key role in modulating microbial colonization, we longitudinally examined the diabetic wound microbiome in tandem with host tissue gene expression. By sequencing 16S ribosomal RNA genes, we show that a longitudinal selective shift in wound microbiota coincides with impaired healing in diabetic mice (Lepr(db/db); db/db). We demonstrate a parallel shift in longitudinal gene expression that occurs in a cluster of genes related to the immune response. Further, we establish a correlation between relative abundance of Staphylococcus spp. and the expression of cutaneous defense response genes. Our data demonstrate that integrating two types of global datasets lends a better under standing to the dynamics governing host-microbe interactions.
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