Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 25, Pages 11567-11572Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1006269107
Keywords
cerebral cortex; dendrites; dendritic spines; long-range connections; global neuronal workspace
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Funding
- Fondation Louis D. of the Institut de France
- European Union [LSHM-CT-2005-512012]
- Unite de Recherche Associee [2182]
- Centre National de la Recherche Scientifique
- Institut Pasteur
- Ministerio de Ciencia e Innovacion [GEN2003-20651-C06-06, BFU2006-13395, SAF2009-09394]
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The neuronal nicotinic acetylcholine receptors (nAChRs) are allosteric membrane proteins involved in multiple cognitive processes, including attention, learning, and memory. The most abundant form of heterooligomeric nAChRs in the brain contains the beta 2- and alpha 4- subunits and binds nicotinic agonists with high affinity. In the present study, we investigated in the mouse the consequences of the deletion of one of the nAChR components: the beta 2-subunit (beta 2(-/-)) on the microanatomy of cortical pyramidal cells. Using an intracellular injection method, complete basal dendritic arbors of 650 layer III pyramidal neurons were sampled from seven cortical fields, including primary sensory, motor, and associational areas, in both beta 2(-/-) and WT animals. We observed that the pyramidal cell phenotype shows significant quantitative differences among different cortical areas in mutant and WT mice. In WT mice, the density of dendritic spines was rather similar in all cortical fields, except in the prelimbic/infralimbic cortex, where it was significantly higher. In the absence of the beta 2-subunit, the most significant reduction in the density of spines took place in this high-order associational field. Our data suggest that the beta 2-subunit is involved in the dendritic morphogenesis of pyramidal neurons and, in particular, in the circuits that contribute to the high-order functional connectivity of the cerebral cortex.
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