Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 26, Pages 11841-11846Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1003953107
Keywords
meiosis; RNA helicase; transposon silencing
Categories
Funding
- EMBL
- Agence National de la Recherche
- National Institutes of Health/National Institute of General Medical Sciences [RO1GM076327]
- Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)
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Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10/1 mutant testes causes loss of DNA methylation and subsequent derepression of retrotransposons in germ cells. The Mov10/1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins.
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