Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 15, Pages 6946-6951Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1002422107
Keywords
chemostat; fluorescence in situ hybridization; mRNA; single cells
Categories
Funding
- NIGMS NIH HHS [GM071508, GM046406, P50 GM071508, GM57071, R37 GM046406, R01 GM046406, R01 GM057071] Funding Source: Medline
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Oscillations in patterns of expression of a large fraction of yeast genes are associated with the metabolic cycle, usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAs from pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
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