Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 108, Issue 3, Pages 1028-1033Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1008856108
Keywords
microarray; laser spectroscopy; tomato plants; aquaporins; carbon nanomaterials
Categories
Funding
- National Institute of Health [R01EB000873, R01CA131164, R01 EB009230, R21CA139373]
- National Science Foundation [DBI-0852737]
- Arkansas Science and Technology Authority (ASTA) [08-CAT-03]
- EPSCoR-NSF-P3 Center [P3-202]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852737] Funding Source: National Science Foundation
- Office Of The Director
- EPSCoR [1003970] Funding Source: National Science Foundation
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Understanding the nature of interactions between engineered nanomaterials and plants is crucial in comprehending the impact of nanotechnology on the environment and agriculture with a focus on toxicity concerns, plant disease treatment, and genetic engineering. To date, little progress has been made in studying nanoparticle-plant interactions at single nanoparticle and genetic levels. Here, we introduce an advanced platform integrating genetic, Raman, photothermal, and photoacoustic methods. Using this approach, we discovered that multiwall carbon nanotubes induce previously unknown changes in gene expression in tomato leaves and roots, particularly, up-regulation of the stress-related genes, including those induced by pathogens and the water-channel LeAqp2 gene. A nano-bubble amplified photothermal/photoacoustic imaging, spectroscopy, and burning technique demonstrated the detection of multiwall carbon nanotubes in roots, leaves, and fruits down to the single nanoparticle and cell level. Thus, our integrated platform allows the study of nanoparticles' impact on plants with higher sensitivity and specificity, compared to existing assays.
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