Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 48, Pages 20810-20815Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1014840107
Keywords
location-guided averaging; nanodomains; total internal reflection fluorescence; single molecules; single particle tracking
Categories
Funding
- National Research Service Award [DK074292]
- Knut and Alice Wallenberg Foundation
- European Molecular Biology Organization
- Japan Society for the Promotion of Science
- National Institutes of Health [MH060600]
Ask authors/readers for more resources
Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane. GFP-labeled versions of both proteins cluster at sites where secretory granules have docked. Single-particle tracking shows that minority populations of both molecules are strongly hindered in their mobility, consistent with their confinement in nanodomains. We measured the fluorescence of granule-associated clusters, the fluorescence of single molecules, and the numbers of unlabeled syntaxin-1 and SNAP-25 molecules per cell. There was a more than 10-fold excess of SNAP-25 over syntaxin-1. Fifty to seventy copies each of syntaxin-1 and SNAP-25 molecules were associated with a single docked granule, many more than have been reported to be required for fusion.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available