Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 8, Pages 3558-3563Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0910172106
Keywords
adipose stem cell; mesenchymal stem cells; pre-adipocytes; iPS cells; metabolism
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Funding
- Japan Society for the Promotion of Science
- National Institutes of Health, Tercel
- Mathers Charitable Foundation
- Fundacion Cellex
- National Institutes of Health [HD027183, DK057978, DK062434, DK063491]
- Helmsley Charitable Trust
- Howard Hughes Medical Institute
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Although adipose tissue is an expandable and readily attainable source of proliferating, multipotent stem cells, its potential for use in regenerative medicine has not been extensively explored. Here we report that adult human and mouse adipose-derived stem cells can be reprogrammed to induced pluripotent stem (iPS) cells with substantially higher efficiencies than those reported for human and mouse fibroblasts. Unexpectedly, both human and mouse iPS cells can be obtained in feeder-free conditions. We discovered that adipose-derived stem cells intrinsically express high levels of pluripotency factors such as basic FGF, TGF beta, fibronectin, and vitronectin and can serve as feeders for both autologous and heterologous pluripotent cells. These results demonstrate a great potential for adipose-derived cells in regenerative therapeutics and as a model for studying the molecular mechanisms of feeder-free iPS generation and maintenance.
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