Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 46, Pages 19748-19753Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1009999107
Keywords
modeling; molecular dynamics; flexible fitting
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB594, SFB646, SFB740, WI3285/1-1]
- European Union and Senatsverwaltung fur Wissenschaft, Forschung und Kultur Berlin (UltraStructureNetwork, Anwenderzentrum)
- Marie Curie International Incoming Fellowship
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Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution. This map, together with a 6.1-angstrom map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model similar to 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
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