Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 107, Issue 17, Pages 7910-7915Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0915163107
Keywords
fluorescence-mediated tomography; molecular imaging; multimodal image fusion; computed tomography
Categories
Funding
- National Institutes of Health [R01 EB006432, P50-CA86355, R24 CA92782, U54 CA126515, UO1 HL08073, R01HL096576]
- American Heart Association [0835623D]
- Regione Veneto and CARIVERONA Foundation
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Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a F-18 isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.
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