Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 51, Pages 21655-21659Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0912384106
Keywords
competition; nucleus; cytoplasm; transport
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Funding
- Beckman Research Institute of City of Hope funds
- National Institutes of Health [HL07470, DK078424]
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Drosha-processed microRNAs (miRNAs) have been shown to be exported from the nucleus to the cytoplasm by Exportin 5, where they are processed a second time to generate mature miRNAs. In this work we show that miRNAs also use CRM1 for nuclear-cytoplasmic shuttling. Inhibition of CRM1 by Leptomycin B results in nuclear accumulation of miRNA guide sequences. Nuclear to cytoplasmic transport can be actively competed by synthetic small interfering RNAs, indicating that this pathway is shared by different classes of processed small RNAs. We also find that CRM1 coimmunoprecipitates with Ago-1, Ago-2, Topo2 alpha, EzH2, and Mta, consistent with a role of Argonautes and small RNAs in chromatin remodeling.
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