Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 22, Pages 8918-8922Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0901471106
Keywords
iPS cells; nonintegrative technique; reprogramming; pluripotency
Categories
Funding
- Swiss National Science Foundation
- Ramon y Cajal program
- Juan de La Cierva program
- G. Harold and Leila Y. Mathers Charitable Foundation, Marato, Terapia Celular
- Fundacion Cellex
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Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors ( retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.
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