Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 27, Pages 10912-10916Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0900975106
Keywords
fluorescence; gold nanoparticle; sensor; conjugated polymer
Categories
Funding
- National Science Foundation Center for Hierarchical Manufacturing at the University of Massachusetts Nanoscale Science and Engineering Center
- National Science Foundation [DMI-0531171]
- National Institutes of Health [GM077173]
- Department of Energy [DE-FG02-04ER46141]
- [AI073425]
- [R01-CA095164]
- [R01-CA105452]
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Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a chemical nose/tongue approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.
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