Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 16, Pages 6860-6865Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0810040106
Keywords
cyanobacteria; transcription
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [16GS0304]
- Nara Institute of Science and Technology
- Grants-in-Aid for Scientific Research [16GS0304] Funding Source: KAKEN
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Although regulation of sigma factors has been intensively investigated, anti-sigma factors have not been identified in oxygenic photosynthetic organisms. A previous study suggested that the sigma factor, SigE, of the cyanobacterium Synechocystis sp. PCC 6803, a positive regulator of sugar catabolism, is posttranslationally activated by light-to-dark transition. In the present study, we found that the H subunit of Mg-chelatase ChlH interacts with sigma factor SigE by yeast two-hybrid screening, and immunoprecipitation analysis revealed that ChlH associates with SigE in a light-dependent manner in vivo. We also found that Mg2+ promotes the interaction of SigE and ChlH and determines their localization in vitro. In vitro transcription analysis demonstrated that ChlH inhibits the transcription activity of SigE. Based on these results, we propose a model in which ChlH functions as an anti-sigma factor, transducing light signals to SigE in a process mediated by Mg2+.
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