Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 44, Pages 18474-18478Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0909149106
Keywords
assembly; biosynthesis
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Funding
- National Institute of General Medical Sciences and the National Center for Research Resources [GM 67626, RR 001209]
- Department of Energy Basic Energy Sciences
- National Institutes of Health National Center for Research Resources Biotechnology
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The P-cluster of nitrogenase is one of the most complex biological metallocenters known to date. Despite the recent advances in the chemical synthesis of P-cluster topologs, the biosynthetic mechanism of P-cluster has not been well defined. Here, we present a combined biochemical, electron paramagnetic resonance, and Xray absorption spectroscopy/extended X-ray absorption fine-structure investigation of the maturation process of P-clusters in Delta nifH molybdenum-iron (MoFe) protein. Our data indicate that the previously identified, [Fe4S4]-like cluster pairs in Delta nifH MoFe protein are indeed the precursors to P-clusters, which can be reductively coupled into the mature [Fe8S7] structures in the presence of Fe protein, MgATP, and dithionite. Moreover, our observation of a biphasic maturation pattern of P-clusters in Delta nifH MoFe protein provides dynamic proof for the previously hypothesized, stepwise assembly mechanism of the two P-clusters in the alpha(2)beta(2)-tetrameric MoFe protein, i.e., one P-cluster is formed in one alpha beta dimer before the other in the second alpha beta dimer.
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