Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 30, Pages 12459-12464Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0902365106
Keywords
microfluidics; nanoparticle; nuclear magnetic resonance
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Funding
- NCI NIH HHS [U54-CA119349, U54 CA119349] Funding Source: Medline
- NHLBI NIH HHS [U01-HL080731, U01 HL080731] Funding Source: Medline
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There is a growing need for fast, highly sensitive and quantitative technologies to detect and profile unaltered cells in biological samples. Technologies in current clinical use are often time consuming, expensive, or require considerable sample sizes. Here, we report a diagnostic magnetic resonance (DMR) sensor that combines a miniaturized NMR probe with targeted magnetic nanoparticles for detection and molecular profiling of cancer cells. The sensor measures the transverse relaxation rate of water molecules in biological samples in which target cells of interest are labeled with magnetic nanoparticles. We achieved remarkable sensitivity improvements over our prior DMR prototypes by synthesizing new nanoparticles with higher transverse relaxivity and by optimizing assay protocols. Wedetected as few as 2 cancer cells in 1-mu L sample volumes of unprocessed fine-needle aspirates of tumors and profiled the expression of several cellular markers in <15 min.
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