Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 25, Pages 10092-10096Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0900914106
Keywords
clinical diagnostic; matrix design; positive/negative ions; ion-formation model; MALDI
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Funding
- International Max Planck Research School
- Max Planck Society
- Ministry of Education of the Czech Republic [LC512]
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The ability to charge huge biomolecules without breaking them apart has made matrix-assisted laser desorption/ionization (MALDI) mass spectrometry an indispensable tool for biomolecular analysis. Conventional, empirically selected matrices produce abundant matrix ion clusters in the low-mass region (< 500 Da), hampering the application of MALDI-MS to metabolomics. An ionization mode of MAILD, a rational protocol for matrix selection based on Bronsted-Lowry acid-base theory and its application to metabolomics, biological screening/profiling/imaging, and clinical diagnostics is illustrated. Numerous metabolites, covering important metabolic pathways (Krebs' cycle, fatty acid and glucosinolate biosynthesis), were detected in extracts, biofluids, and/or in biological tissues (Arabidopsis thaliana, Drosophila melanogaster, Acyrthosiphon pisum, and human blood). This approach moves matrix selection from black art'' to rational design and sets a paradigm for small-molecule analysis via MALDI-MS.
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