Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 16, Pages 6730-6735Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0900969106
Keywords
antigen processing; cross-presentation; endocytosis; Fc receptor; MHC class I
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Funding
- Dutch Cancer Society [UL 2004-3008]
- Netherlands Organization for Scientific Research
- EU [LSHC-CT-2006-518234, LSHB-CT-2004-512074]
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Dendritic cells (DCs) are crucial for priming of naive CD8(+) T lymphocytes to exogenous antigens, so-called cross-priming.'' We report that exogenous protein antigen can be conserved for several days in mature DCs, coinciding with strong cytotoxic T lymphocyte cross-priming potency in vivo. After MHC class I peptide elution, protein antigen-derived peptide presentation is efficiently restored, indicating the presence of an intracellular antigen depot. We characterized this depot as a lysosome-like organelle, distinct from MHC class II compartments and recently described early endosomal compartments that allow acute antigen presentation in MHC class I. The storage compartments we report here facilitate continuous supply of MHC class I ligands. This mechanism ensures sustained cross-presentation by DCs, despite the short-lived expression of MHC class I-peptide complexes at the cell surface.
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