Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 35, Pages 14867-14871Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0904385106
Keywords
density functional theory; hybrid potentials; iron sulfur cluster; redox chemistry; S-adenosyl-L-methionine
Categories
Funding
- French Agence Nationale pour la Recherche [ANR-08-BLAN-0224-01]
- Commissariat a l'Energie Atomique et le Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0224] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
Radical S-adenosine-L-methionine (SAM or AdoMet) proteins are involved in chemically difficult reactions including the synthesis of cofactors, the generation of protein radicals, and the maturation of complex organometallic catalytic sites. In the first and common step of the reaction, a conserved [Fe4S4] cluster donates an electron to perform the reductive cleavage of AdoMet into methionine and a reactive radical 5'-dA. species. The latter extracts a hydrogen atom from substrate eliciting one of the about 40 reactions so far characterized for this family of proteins. It has been suggested that the radical-generating mechanism differs depending on whether AdoMet is a cofactor or a substrate. It has also been speculated that electron transfer from the [Fe4S4] cluster to AdoMet is sulfur-based. Here we have used protein crystallography and theoretical calculations to show that regardless whether AdoMet serves as a cofactor or a substrate, the 5'-dA. generating mechanism should be common to the radical SAM proteins studied so far, and that electron transfer is mediated by a unique Fe from the conserved [Fe4S4] cluster. This unusual electron transfer is determined by the sulfonium ion in AdoMet.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available