Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 11, Pages 4549-4554Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0810280106
Keywords
abscisic acid; aminoacyl tRNA protein transferase; lipid bodies; targeted protein degradation
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Funding
- Lawes Trust
- Biotechnology and Biological Sciences Research Council (BBSRC) studentship
- National Fruit and Cider Institute PhD studentship
- University of Nottingham (Nottingham, U.K.)
- Centre for Plant Integrative Biology (CPIB)
- Deutsche Forschungsgemeinschaft [BA 1158/3/1]
- BBSRC [BBS/E/C/00004948] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004948] Funding Source: researchfish
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The N-end rule pathway targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Two components of this pathway in Arabidopsis thaliana, PROTEOLYSIS6 (PRT6) and arginyl-tRNA: protein arginyltransferase ( ATE), were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. Sensitivity of prt6 mutant seeds to ABA inhibition of endosperm rupture reduced with after-ripening time, suggesting that seeds display a previously undescribed window of sensitivity to ABA. Reduced root growth of prt6 alleles and the ate1 ate2 double mutant was rescued by exogenous sucrose, and the breakdown of lipid bodies and seed-derived triacylglycerol was impaired in mutant seedlings, implicating the N-end rule pathway in control of seed oil mobilization. Epistasis analysis indicated that PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. The N-end rule pathway of protein turnover is therefore postulated to inactivate as-yet unidentified key component(s) of ABA signaling to influence the seed-to-seedling transition.
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