Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 106, Issue 34, Pages 14687-14692Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0904664106
Keywords
calcium; fast inactivation; STIM1
Categories
Funding
- National Institutes of Health [DE12309, DK38938]
- Ruth S. Harrell Professorship in Medical Research
- National Institute on Drug Abuse [DA00266, DA10309]
- National Institute of Mental Health [MH068830]
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Ca2+ influx by store-operated Ca2+ influx channels (SOCs) mediates many cellular functions regulated by Ca2+, and excessive SOC-mediated Ca2+ influx is cytotoxic and associated with disease. One form of SOC is the CRAC current that is mediated by Orai channels activated by STIM1. A fundamental property of the native CRAC and of the Orais is fast Ca2+-dependent inactivation, which limits Ca2+ influx to guard against cellular damage. The molecular mechanism of this essential regulatory mechanism is unknown. We report here the fast Ca2+-dependent inactivation is mediated by three conserved glutamates in the C termini (CT) of Orai2 and Orai3, which show prominent fast Ca2+-dependent inactivation compared with Orai1. Transfer of the CT between the Orais transfers both the extent of channel opening and the mode of fast Ca2+-dependent inactivation. Fast Ca2+-dependent inactivation of the Orais also requires a domain of STIM1; fragments of STIM1 that efficiently open Orai channels do not evoke fast inactivation unless they include an anionic sequence that is C-terminal to the STIM1-Orai activating region (SOAR). Our studies suggest that Orai CT are necessary and sufficient to control pore opening and uncover the molecular mechanism of fast Ca2+-dependent inactivation that has implications for Ca2+ influx by SOC in physiological and pathological states.
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