Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 40, Pages 15517-15522Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0807841105
Keywords
B lymphocyte; BAFF; immune memory; mouse
Categories
Funding
- U.S. Public Health Service [A1054488, A1073939, A143603, T32 A1-055428]
- Lupus Foundation of America
- Dermatology and Arthritis Foundations
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We have used an inhibiting antibody to determine whether preimmune versus antigen-experienced B cells differ in their requisites for BLyS, a cytokine that controls differentiation and survival Whereas in vivo BLyS inhibition profoundly reduced naive B cell numbers and primary immune responses, it had a markedly smaller effect on memory B cells and long-lived plasma cells, as well as secondary immune responses. There was heterogeneity within the memory pools, because IgM-bearing memory cells were sensitive to BLyS depletion whereas IgG-bearing memory cells were not, although both were more resistant than naive cells. There was also heterogeneity within B1 pools, as splenic but not peritoneal B1 cells were diminished by anti-BLyS treatment, yet the number of natural antibody-secreting cells remained constant. Together, these findings show that memory B cells and natural antibody-secreting cells are BLyS-independent and suggest that these pools can be separately manipulated.
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