Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 42, Pages 16398-16403Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0808463105
Keywords
BiP2; bZIP28; endoplasmic reticulum; signal transduction; thermotolerance
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Funding
- Michigan State University-Department of Energy Plant Research Laboratory group project [DE-FG02-91 ER20021]
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In plants, heat stress responses are controlled by heat stress transcription factors that are conserved among all eukaryotes and can be constitutively expressed or induced by heat. Heat-inducible transcription factors that are distinct from the classical heat stress transcription factors have also been reported to contribute to heat tolerance. Here, we show that bZIP28, a gene encoding a putative membrane-tethered transcription factor, is up-regulated in response to heat and that a bZIP28 null mutant has a striking heat-sensitive phenotype. The heat-inducible expression of genes that encode BiP2, an endoplasmic reticulum (ER) chaperone, and HSP26.5-P, a small heat shock protein, is attenuated in the bZIP28 null mutant. An estradiol-inducible bZIP28 transgene induces a variety of heat and ER stress-inducible genes. Moreover, heat stress appears to induce the proteolytic release of the predicted transcription factor domain of bZIP28 from the ER membrane, thereby causing its redistribution to the nucleus. These findings indicate that bZIP28 is an essential component of a membrane-tethered transcription factor-based signaling pathway that contributes to heat tolerance.
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