Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 25, Pages 8580-8584Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0804424105
Keywords
genome integrity; transcription; BLM; WRN
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Funding
- Cancer Research UK Funding Source: Medline
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Although the active forms of factors involved in DNA-related processes such as DNA replication, repair, and transcription are associated with chromatin, proteins are rarely purified from this source. Here, we describe a protocol for the isolation of chromatin-associated factors and use it to identify proteins interacting with human RNA polymerase II (RNAPII). Our data establish RECQ5 helicase as a bona fide RNAPII-associated protein. The RECQ5-RNAPII interaction is direct and is mediated by the RPB1 subunit of RNAPII, and RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII These data suggest an unexpected role for RECQ5 helicase at the interface of transcription and genomic stability.
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