Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 43, Pages 16665-16670Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0807104105
Keywords
Caenorhabditis elegans operon; RNA polymerase II C-terminal domain; SRp20; CTD
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Funding
- National Institute of General Medical Science [GM42432, GM47869]
- Howard Hughes Medical Institute
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RNA polymerase II (Pol II) transcription termination involves two linked processes: mRNA T-end formation and release of Pol II from DNA. Signals for 3' processing are recognized by a protein complex that includes cleavage polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF). Here we identify suppressors encoding proteins that play roles in processes at the 3' ends of genes by exploiting a mutation in which the 3' end of another gene is transposed into the first gene of the Calenorhabditis elegans lin-15 operon. As expected, genes encoding CPSF and CstF were identified in the screen. We also report three suppressors encoding proteins containing a domain that interacts with the C-terminal domain of Pol II (CID). We show that two of the CID proteins are needed for efficient 3'cleavage and thus may connect transcription termination with RNA cleavage. Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3'-end cleavage, leading to termination of transcription.
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