Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 51, Pages 20404-20409Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0811011106
Keywords
in vivo DNA assembly; genome synthesis; combinatorial assembly; yeast transformation; Mycoplasma genitalium; synthetic biology
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Funding
- Synthetic Genomics, Inc
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We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
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