Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 48, Pages 18776-18781Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0800831105
Keywords
adeno-associated virus; cochlea; gap junctions; gene transfer; hearing loss
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Funding
- Fondazione Cariparo, Padua, Italy
- Telethon Italy [GGP05131]
- European Commission [LSHG-CT-20054-512063]
- National Institutes of Health/National Institute of Dental and Craniofacial Research
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Connexin 26 (Cx26) and connexin 30 (COO) are encoded by two genes (GJB2 and GJa6, respectively) that are found within 50 kb in the same complex deafness locus, DFNB1. Immunocytochemistry and quantitative PCR analysis of Cx30 KO mouse cultures revealed that Cx26 is downregulated at the protein level and at the mRNA level in nonsensory cells located between outer hair cells and the stria vascularis. To explore connexin coregulation, we manipulated gene expression using the bovine adeno-associated virus. Overexpression of Cx30 in the Cx30 KO mouse by transduction with bovine adeno-associated virus restored Cx26 expression, permitted the formation of functional gap junction channels, and rescued propagating Ca2+ signals. Ablation of Cx26 by transduction of Cx26(loxP/loxP) cultures with a Cre recombinase vector caused concurrent downregulation of Cx30 and impaired intercellular communication. The coordinated regulation of Cx26 and Cx30 expression appears to occur as a result of signaling through PLC and the NF-kappa B pathway, because activation of IP3-mediated Ca2+ responses by stimulation of P2Y receptors for 20 min with 20 nM ATP increased the levels of Cx26 transcripts in Cx30 KO cultures. This effect was inhibited by expressing a stable form of the I kappa B repressor protein that prevents activation/translocation of NF-kappa B. Thus, our data reveal a Ca2+-dependent control in the expression of inner ear connexins implicated in hereditary deafness as well as insight into the hitherto unexplained observation that some deafness-associated DFNB1 alleles are characterized by hereditable reduction of both GJB2 and GJB6 expression.
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