Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 105, Issue 4, Pages 1182-1185Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0711539105
Keywords
colloidal gold; molecular motor; motility
Categories
Funding
- NIAID NIH HHS [R37 AI016478, R01 AI016478, AI016478] Funding Source: Medline
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Flagellated bacteria, such as Escherichia coli, are propelled by helical flagellar filaments, each driven at its base by a reversible rotary motor, powered by a transmembrane proton flux. Torque is generated by the interaction of stator proteins, MotA and MotB, with a rotor protein FliG. The physiology of the motor has been studied extensively in the regime of relatively high load and low speed, where it appears to operate close to thermodynamic equilibrium. Here, we describe an assay that allows systematic study of the motor near zero load, where proton translocation and movement of mechanical components are rate limiting. Sixty-nanometer-diameter gold spheres were attached to hooks of cells lacking flagellar,filaments, and light scattered from a sphere was monitored at the image plane of a microscope through a small pinhole. Paralyzed motors of cells carrying a motA point mutation were resurrected at 23 degrees C by expression of wild-type MotA, and speeds jumped from zero to a maximum value (approximate to 300 Hz) in one step. Thus, near zero load, the speed of the motor is independent of the number of torque-generating units. Evidently, the units act independently (they do not interfere with one another), and there are no intervals during Which a second unit can add to the speed generated by the first (the duty ratio is close to 1).
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