Journal
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE
Volume 227, Issue H1, Pages 50-57Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0954411912458739
Keywords
Bone scaffold; fused deposition modeling; freeze-drying; beta-tricalcium phosphate; prefreezing temperature; alkaline phosphatase
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Funding
- Shanghai Innovation Foundation [09YZ34]
- China Postdoctoral Science Foundation [20100470110]
- Laboratory of Manufacturing Automation and Robotics [ZK1103]
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A combined method of tricalcium phosphate (TCP) scaffold production, which comprised negative mold and scaffold fabrication, was reported in this study. The negative mold structure was designed by computer and fabricated by fused deposition modeling (FDM) technology, while the TCP scaffold was produced by freeze-drying technology under different prefreezing temperatures of 10 degrees C, 30 degrees C, and 86 degrees C and thermal treatment to get beta-TCP. The scaffold structure was evaluated with X-ray, scanning electron microscopy (SEM), compressive mechanical testing, and micro-computerized tomography (micro-CT). The cell-scaffold interaction was studied by culturing dog bone marrow stromal cells (BMSCs) on the scaffolds and assessing differentiated BMSC function by measuring cellular alkaline phosphatase (ALP) activity. The results showed good interconnectivity and good pore distribution with the pore size ranging from 50 to 250 mu m and compressive modulus of 1.18 MPa at a prefreezing temperatures of -10 degrees C. In vitro cell culture results indicated that the porous scaffolds were not toxic to bone cells. These results demonstrate that rapid prototyping and freeze-drying technologies for creating beta-TCP scaffolds are promising for bone tissue engineering.
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