Journal
PRENATAL DIAGNOSIS
Volume 33, Issue 3, Pages 214-222Publisher
WILEY-BLACKWELL
DOI: 10.1002/pd.4045
Keywords
-
Categories
Funding
- Japan Society for the Promotion of Science KAKENHI [23592406, 24791712, 22591827]
- Grants-in-Aid for Scientific Research [25462563, 23592406, 24791711, 22591827] Funding Source: KAKEN
Ask authors/readers for more resources
Objective The aim of this study was to characterize placenta-specific microRNAs in fetal growth restriction (FGR) pregnancy. Method Placenta-specific miRNAs were identified by next-generation sequencing analysis. Subsequently, quantitative real-time reverse-transcription polymerase chain reaction was used to identify FGR placenta-specific miRNAs whose level of expression was significantly decreased in FGR placenta (n=45) compared with uncomplicated placenta (n=50). FGR pregnancy-associated, placenta-specific microRNAs were identified in maternal plasma after delivery at significantly decreased concentrations, and their circulating levels in maternal plasma was compared between FGR pregnancies (n=10) and uncomplicated pregnancies (n=10). Results Out of the ten placenta-specific microRNAs that we identified, seven placenta-specific microRNAs (hsa-miR-518b, hsa-miR-1323, hsa-miR-516b, hsa-miR-515-5p, hsa-miR-520h, hsa-miR-519d, and hsa-miR-526b) from the chromosome 19 microRNA cluster were identified as FGR placenta-specific microRNAs. Four FGR placenta-specific microRNAs (hsa-miR-518b, hsa-miR-1323, hsa-miR-520h, and hsa-miR-519d) were confirmed as FGR pregnancy-associated, placenta-specific miRNAs, but their circulating levels in maternal plasma showed no significant differences between FGR pregnancy and uncomplicated pregnancy. Conclusion Our data suggest that reduced expression in placenta of certain FGR placenta-specific miRNAs is associated with FGR and that the discrepancy between expression in FGR placenta and their circulating levels in maternal plasma will be crucial to understanding how placenta-specific microRNAs are released into the maternal circulation. (c) 2013 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available